Endoglin antibody

Catalog No. ABIN319844

Product Details anti-Endoglin Antibody

Target: Endoglin (ENG)

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the CD105 cell surface antigen.

Purification: Affinity Chromatography on Protein G

Immunogen: Partially purified cell membrane antigens from fresh leukemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line.

Clone: SN6

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 / Endoglin (ENG Products)

Background: CD105 is a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukaemia cells.Synonyms: END, ENG, HHT1, ORW, ORW1

Gene ID: 9606

UniProt: P17813