Endoglin antibody (FITC)
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- Target See all Endoglin (ENG) Antibodies
- Endoglin (ENG)
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Reactivity
- Human, Horse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Endoglin antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- SM1177F recognizes the CD105 cell surface antigen.
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- Partially purified cell membrane antigens from fresh leukaemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
- Clone
- SN6
- Isotype
- IgG1
- Top Product
- Discover our top product ENG Primary Antibody
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- Application Notes
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Flow cytometry (use 10 μL of neat antibody to label 10^6 cells in 100 μL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as a preservative and 1 % BSA as a stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Endoglin (ENG)
- Alternative Name
- CD105 / Endoglin (ENG Products)
- Background
- CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2. CD105 is a glycoprotein homodimer of 95 kD subunits. CD105 is expressed by endothelial cells, activated monocytes and some leukaemia cells.Synonyms: END, ENG, HHT1, ORW, ORW1
- Gene ID
- 9606
- UniProt
- P17813
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