The antibody recognizes the human CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11 (see ref. 4). The use of SM1092LE is recommended for this purpose.
FCGR2A
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
3E8
unconjugated
Application Notes
Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
Human CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II