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CD16 antibody

CD16 Reactivity: Human FACS, IP Host: Mouse Monoclonal LNK16 unconjugated

Catalog No. ABIN319691

Datasheet as PDF

Target See all CD16 Antibodies

CD16
Reactivity
133

29

20

20

20

9

8

8

8

4

3

3

2

2

2

2

2

2

2

2

2

2
Human
Host
115

26

20

1
Mouse
Compatible Secondaries
Clonality
136

24

1
Monoclonal
Conjugate
64

18

14

13

7

5

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD16 antibody is un-conjugated
Application
133

36

28

28

26

24

23

17

6

6

5

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity

This antibody recognizes CD16. This antibody has been reported to block the binding of IgG to the CD16 Molecule. (4)

Purification

Ion Exchange Chromatography

Immunogen

Normal Human peripheral blood granulocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Clone

LNK16

Isotype

IgG1

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CD16 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal LNK16 FITC

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CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MEM-168 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS, IP, Func, WB Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody (Biotin)
CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 Biotin

anti-CD16 (CD16) (AA 17-208) antibody
CD16 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 2G10A9 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP, Func, IHC (fro) Host: Mouse Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS Host: Mouse Monoclonal GRM1 unconjugated

Application Notes

Flow cytometry: Use 10 μL of 1/10-1/20 diluted antibody to label 10^6 cells or 100 μL human whole blood. Immunoprecipitation. Does not work in Immunohistochemistry on Frozen and Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD16

Alternative Name

CD16 (CD16 Products)

Background

CD16 is a 50-65kD cell surface molecule which exists in two forms, a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID

2214

NCBI Accession

NP_000560

UniProt

P08637

Pathways

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process