CD44 antibody (FITC)

Catalog No. ABIN319579

Product Details anti-CD44 Antibody (FITC)

Target: CD44

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Ly24.2 found on more than 90 % bone marrow cells and spleen cells, 56 % of thymus cells and 90 % of lymph node cells. This antibody recognises the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice. Spleen cells from immunised mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.

Clone: 5035-41-1D

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Target Details for CD44

Target: CD44

Alternative Name: CD44 (CD44 Products)

Background: CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 12505

NCBI Accession: NP_001034240

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling