ITGAL antibody (FITC)

Catalog No. ABIN317411

Product Details anti-ITGAL Antibody (FITC)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Pig, Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGAL antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody is specific for the porcine CD11a cell surface antigen, which is expressed on a range of cells including peripheral blood mononuclear cells, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (1).

Characteristics: Synonyms: Integrin alpha-L, LFA1, LFA-1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocytefunction-associated molecule 1 alpha chain, CD11 antigen-like family member A

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Porcine alveolar macrophages.

Clone: BL1H8

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. Functional Assays: Removal of Sodium Azide is recommended prior to use.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a / ITGAL (ITGAL Products)

Background: CD11a is a member of the integrin family of cell adhesion molecules. It is a glycoprotein expressed in combination with the CD18 beta chain. The complex is a member of the beta 2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM2, ICAM3. CD11a is expressed on thymocytes, T and B lymphocytes, granulocytes, monocytes, and macrophages.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gene ID: 281874

UniProt: P61625

Pathways: Activated T Cell Proliferation, Integrin Complex