EGLN3 antibody (C-Term)

Catalog No. ABIN311509

Product Details anti-EGLN3 Antibody

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FWSDRRNPHE VQPSYATRYA MTVWYFDAEE RAEAKKKFRN LTRKTESALT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 77%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against EGLN3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGLN3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 239 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3 (EGLN3 Products)

Background: EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
Alias Symbols: FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3
Protein Interaction Partner: FOXJ2, SERTAD1, NCAPH2, EFHC2, ABI2, SIAH2, REL, HIF1A, TTC23L, SQSTM1, HEXIM1, IKBKG, IKBKB, CHUK, MAPK7, MAPK6, MAPK1, PKM, EPAS1, UBD, MAGEA11, UBC, ATF4, WTIP, AJUBA, LIMD1, SPRY2, ADRB2, PRPF19, Myog, EGLN3, EGLN2, EGLN1, OS9, HIF3A, WDR83,
Protein Size: 239

Molecular Weight: 27 kDa

Gene ID: 112399

NCBI Accession: NM_022073, NP_071356

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity