EIF2S3 antibody (N-Term)

Catalog No. ABIN310758

Product Details anti-EIF2S3 Antibody

Target: EIF2S3 (Eukaryotic Translation Initiation Factor 2, Subunit 3 Gamma, 52kDa (EIF2S3))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LDDPSCPRPE CYRSCGSSTP DEFPTDIPGT KGNFKLVRHV SFVDCPGHDI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF2S3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF2S3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 472 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF2S3

Target: EIF2S3 (Eukaryotic Translation Initiation Factor 2, Subunit 3 Gamma, 52kDa (EIF2S3))

Alternative Name: EIF2S3 (EIF2S3 Products)

Background: EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Alias Symbols: EIF2, EIF2G, EIF2gamma, eIF-2gA
Protein Interaction Partner: UBC, RNF2, rev, CCAR2, PLD3, EEF1E1, AIMP1, AIP, IQGAP1, RARS, QARS, IMPDH2, DNAJA1, EIF2S1, MAPK6, TARDBP, FN1, UBD, GRK5, CAND1, CDK2, SUMO1, ELAVL1, SUMO2, NDRG1, RAD21, HDGF, DCC, METAP2, EIF1B, CASP4, H3F3A, CTCF,
Protein Size: 472

Molecular Weight: 51 kDa

Gene ID: 1968

NCBI Accession: NM_001415, NP_001406

UniProt: P41091