CYBB antibody (C-Term)

Catalog No. ABIN310582

Product Details anti-CYBB Antibody

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YGRPNWDNEF KTIASQHPNT RIGVFLCGPE ALAETLSKQS ISNSESGPRG

Predicted Reactivity: Cow: 79%, Dog: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CYBB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CY24B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 570 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: CYBB (CYBB Products)

Background: Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase, neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Alias Symbols: CGD, NOX2, IMD34, AMCBX2, GP91-1, GP91PHOX, p91-PHOX, GP91-PHOX,
Protein Interaction Partner: IQGAP1, ACTB, UBC, SUMO1, RAC1, NCF4, NCF2, NCF1, CYBA, ACTG1,
Protein Size: 570

Molecular Weight: 62 kDa

Gene ID: 1536

NCBI Accession: NM_000397, NP_000388

UniProt: P04839