ALOX15B antibody (C-Term)

Catalog No. ABIN310247

Product Details anti-ALOX15B Antibody

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow, Horse, Rabbit, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ATCEGFIATL PPVNATCDVI LALWLLSKEP GDQRPLGTYP DEHFTEEAPR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against ALOX15B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ALOX15B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 676 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALOX15B antibody (ABIN310247)

Subbarayan, Xu, Kim, Yang, Hoque, Sabichi, Llansa, Mendoza, Logothetis, Newman, Lippman, Menter: "Inverse relationship between 15-lipoxygenase-2 and PPAR-gamma gene expression in normal epithelia compared with tumor epithelia." in: Neoplasia (New York, N.Y.), Vol. 7, Issue 3, pp. 280-93, (2005) (PubMed).

Target Details for ALOX15B

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Alternative Name: ALOX15B (ALOX15B Products)

Background: ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively.This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Alias Symbols: 15-LOX-2
Protein Interaction Partner: RXRA, PPARG, ERAL1,
Protein Size: 676

Molecular Weight: 74 kDa

Gene ID: 247

NCBI Accession: NM_001141, NP_001132

UniProt: O15296