Renin antibody (C-Term)

Catalog No. ABIN310144

Product Details anti-Renin Antibody

Target: Renin (REN)

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Sheep, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Renin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YSSKKLCTLA IHAMDIPPPT GPTWALGATF IRKFYTEFDR RNNRIGFALA

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against REN. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human REN

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Renin antibody (ABIN310144)

Kumai, Bernier, Perry: "Angiotensin-II promotes Na+ uptake in larval zebrafish, Danio rerio, in acidic and ion-poor water." in: The Journal of endocrinology, Vol. 220, Issue 3, pp. 195-205, (2014) (PubMed).

Target Details for Renin

Target: Renin (REN)

Alternative Name: REN (REN Products)

Background: Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Mutations in this gene have been shown to cause familial hyperproreninemia.Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.
Alias Symbols: FLJ10761, HNFJ2
Protein Interaction Partner: AGT, ATP6AP2, KCTD15, PCSK5, RENBP, PCSK1, M6PR, CTSB,
Protein Size: 406

Molecular Weight: 45 kDa

Gene ID: 5972

NCBI Accession: NM_000537, NP_000528

UniProt: P00797

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour