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CD4 antibody (Biotin)

CD4 Reactivity: Rat FACS, IHC, IHC (fro), IHC (p), CyTox Host: Mouse Monoclonal W3-25 Biotin

Catalog No. ABIN307931

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Reactivity
336

209

121

48

24

20

19

18

17

10

10

10

10

10

10

9

9

9

8

5

4

2

1

1

1

1

1
Rat
Host
353

147

113

3

3

1
Mouse
Compatible Secondaries
Clonality
474

138

5
Monoclonal
Conjugate
205

69

62

44

35

12

11

10

9

9

9

9

8

8

7

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
This CD4 antibody is conjugated to Biotin
Application
498

191

127

102

93

69

69

42

39

34

31

29

10

8

8

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Cytotoxicity Test (CyTox)

Specificity

Anti-rat CD4 monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95 % ), a subset of peripheral T cells and peritoneal macrophages. (1,2,3, 10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48000-52,00.

Purification

Protein G purified

Immunogen

Rat thymocyte membrane.

Clone

W3-25

Isotype

IgG1

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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Ctox, Flo (0.5 μg/10 x10⁶), IHC, IHC-Fr, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.02 % sodium azide, EIA grade BSA.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freeze thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

Name/Gene ID: CD4

Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule

Gene ID

920

UniProt

P01730

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events