BrdU antibody (FITC)

Catalog No. ABIN306900

Product Details anti-BrdU Antibody (FITC)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Characteristics: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.

Purification: Protein A/G Chromatography

Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin was used to immunize BALB/c mice

Clone: MoBU-1

Isotype: IgG1

Application Details

Application Notes: Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern. Use at 1-5 μg/106 cells for flow cytometry. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (bromodeoxyuridine) (BrdU Products)

Target Type: Chemical