This antibody can be used for Western blotting (1-2 ug/ml), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 ug/ml) and flow cytometry. Positive Control: Antibodies labeled with FITC.
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversation of fluorescein-labeled detection into immunoenzymatic assays.