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Phosphotyrosine antibody (Peroxidase (POD))

WB, IP, IHC, EIA Host: Mouse Monoclonal PY20 Peroxidase (POD)

Catalog No. ABIN306702

Datasheet as PDF

Target See all Phosphotyrosine products

Phosphotyrosine

Reactivity

Please inquire
Host
84

28

1
Mouse
Compatible Secondaries
Clonality
95

18
Monoclonal
Conjugate
36

17

10

8

4

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is conjugated to Peroxidase (POD)
Application
79

59

39

38

32

22

21

17

13

9

7

7

7

6

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Characteristics

Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate,The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.

Purification

Prot A/G

Immunogen

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Clone

PY20

Isotype

IgG2b
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Atto 594

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 PE

Application Notes

Application: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.

Restrictions

For Research Use only
Buffer

Provided in 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide

Storage

-20 °C

Storage Comment

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target

Phosphotyrosine

Abstract

Phosphotyrosine Products

Target Type

Amino Acid