Phosphoserine antibody

Catalog No. ABIN305423

Product Details anti-Phosphoserine Antibody

Target: Phosphoserine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificity: Recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not cross react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross reactivity with free phosphothreonine is observed. Phosphorylation and dephosphorylation of intracellular proteins occurs in response to extracellular signals. In certain disease states such as cancer, abnormal protein phosphorylation can occur.

Purification: Purified

Immunogen: Keyhole Limpet Hemocyanin - phosphoserine conjugates.

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:2000), IP, WB

Usage: Western Blot: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10 mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide, 50 % gelatin/glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid