CD3 antibody

Catalog No. ABIN305267

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Purification: Ammonium sulfate precipitation

Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

Type of Immunogen: Cells

Clone: 1F4

Isotype: IgM

Application Details

Application Notes: Approved: Flo (1:10 - 1:25), IHC, IHC-Fr (1:10 - 1:25), IHC-P (1:10), IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway