For best experience we recommend to activate Javascript in your browser.

CD16 antibody (Biotin)

CD16 Reactivity: Human FACS Host: Mouse Monoclonal MEM-154 Biotin

Catalog No. ABIN303523

Datasheet as PDF

Target See all CD16 Antibodies

CD16
Reactivity
133

29

20

20

20

9

8

8

8

4

3

3

2

2

2

2

2

2

2

2

2

2
Human
Host
115

26

20

1
Mouse
Compatible Secondaries
Clonality
136

24

1
Monoclonal
Conjugate
64

18

14

13

7

5

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD16 antibody is conjugated to Biotin
Application
133

36

28

28

26

24

23

17

6

6

5

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.

Immunogen

Human granulocytes

Clone

MEM-154

Isotype

IgG1

Top Product

Discover our top product CD16 Primary Antibody
anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP Host: Mouse Monoclonal LNK16 unconjugated

anti-CD16 (CD16) antibody (FITC)
CD16 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal LNK16 FITC

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MEM-168 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS, IP, Func, WB Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody (Biotin)
CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 Biotin

anti-CD16 (CD16) (AA 17-208) antibody
CD16 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 2G10A9 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP, Func, IHC (fro) Host: Mouse Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS Host: Mouse Monoclonal GRM1 unconjugated

Application Notes

Flow cytometry: starting point for use in indirect immunoflourescence is 1:500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date

12 months
Target

CD16

Alternative Name

CD16 (CD16 Products)

Background

CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID

2214

UniProt

P08637

Pathways

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process