Nestin antibody (AA 1484-1500)

Catalog No. ABIN303340

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 1484-1500

Reactivity: Human, Mouse, Rat, Dog, Cow, Hamster, Rabbit, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Nestin.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Dog, Hamster, Macaque, Rabbit, RatSpecies reactivity (tested):Human, Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin.

Application Details

Application Notes: ELISA (1/20000-1/80000). Immunofluorescence. Western Blot (1/500-1/3000). Immunohistochemistry on Paraffin Sections (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170

Gene ID: 10763

NCBI Accession: NP_006608

UniProt: P48681