TNFRSF10A antibody (PE)

Catalog No. ABIN302032

Product Details anti-TNFRSF10A Antibody (PE)

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF10A antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.

Clone: DR-4-02

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-5 μg/mL.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-TNFRSF10A antibody (PE) (ABIN302032)

Vondálová Blanárová, Jelínková, Szöor, Skender, Soucek, Horváth, Vaculová, Andera, Sova, Szöllosi, Hofmanová, Vereb, Kozubík: "Cisplatin and a potent platinum(IV) complex-mediated enhancement of TRAIL-induced cancer cells killing is associated with modulation of upstream events in the extrinsic apoptotic pathway." in: Carcinogenesis, Vol. 32, Issue 1, pp. 42-51, (2010) (PubMed).

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Target Details for TNFRSF10A

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: CD261 / TRAIL-R1 (TNFRSF10A Products)

Synonyms: APO2 antibody, CD261 antibody, DR4 antibody, TRAILR-1 antibody, TRAILR1 antibody, TNFRSF10A antibody, TNF receptor superfamily member 10a antibody, tumor necrosis factor receptor superfamily member 26 antibody, tumor necrosis factor receptor superfamily, member 10a antibody, TNFRSF10A antibody, LOC510798 antibody

Background: TNF receptor superfamily member 10a,TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).,DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1

Gene ID: 8797

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity