NLRP3 antibody (AA 82-93)

Catalog No. ABIN297366

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: AA 82-93

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NLRP3. This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bat, Panda, Pig (92%) Dog, Bovine, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bat, Panda, Pig (92%), Dog, Bovine, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NALP3 / NLRP3 (NLRP3 Products)

Background: Name/Gene ID: NLRP3

Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS

Gene ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001230062, NP_001120933, NP_001120934

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome