CXCR7 antibody (AA 100-150)

Catalog No. ABIN295878

Product Details anti-CXCR7 Antibody

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Binding Specificity: AA 100-150

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Pig, Horse, Bat, Hamster, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: A portion of amino acids 100-150 of human RDC1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum (100%) Platypus, Xenopus (92%) Turkey (83%).

Purification: Protein G purified

Immunogen: A synthetic peptide (QHNQWPMGELTC) corresponding amino acids 106-117 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum (100%), Platypus, Xenopus (92%), Turkey (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1 - 10 μg/mL), IHC, IHC-P (10 μg/mL), WB (4 - 6 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine buffer, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Alternative Name: ACKR3 / CXCR7 (CXCR7 Products)

Background: Name/Gene ID: ACKR3
Subfamily: Chemokine
Family: GPCR

Synonyms: ACKR3, Chemokine orphan receptor 1, CMKOR1, CXCR7, CXC-R7, GPRN1, RDC1, RDC-1, CXCR-7, G-protein coupled receptor 159, GPR159

Gene ID: 57007

NCBI Accession: NP_064707

UniProt: P25106

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling