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GFP antibody (Alkaline Phosphatase (AP))

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Goat Polyclonal Alkaline Phosphatase (AP)

Catalog No. ABIN294471

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
173

15

11

9
Aequorea victoria
Host
64

62

33

13

9

5

2

1
Goat
Compatible Secondaries
Clonality
112

72
Polyclonal
Conjugate
81

12

11

10

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Alkaline Phosphatase (AP)
Application
146

74

46

32

27

25

23

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.

Purification

Immunoaffinity purified

Immunogen

GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
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anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA (1:16000 - 1:32000), IHC, WB

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:16000-1:32000, 1.0 g of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Aequorea victoria

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freezing

Storage

4 °C

Storage Comment

4°C, avoid freezing.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Name/Gene ID: GFP

Synonyms: GFP

UniProt

P42212