Interleukin 17a antibody

Catalog No. ABIN290358

Product Details anti-Interleukin 17a Antibody

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Neutralization (Neut), Immunocytochemistry (ICC)

Specificity: Recognizes human IL-17.

Purification: Immunoaffinity purified

Immunogen: Purified, E. coli-derived, recombinant human interleukin 17.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo, ICC (2 - 15 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA. Neutralization: The ND50 is ~0.1-0.3 μg/mL in the presence of 25 ng/mL of rhIL-17, using the NHDF cell line. Immunocytochemistry: 2-15 μg/mL. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 0.2 ng/well. In this format, this antibody shows approximately 10 % cross-reactivity with rmIL-17 and rhIL-17F and less than 1 % cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D and rhIL-17E. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 5 ng/lane under both non-reducing and reducing conditions. Flow Cytometry: Tested in PBMCs. For intracellular staining to detect IL-17, cells must be first fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled polyclonal antibodies can be visualized by a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A / IL17 (IL17A Products)

Background: Name/Gene ID: IL17A
Family: Interleukin

Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17

Gene ID: 3605

UniProt: Q16552