Vimentin antibody

Catalog No. ABIN271977

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Vimentin protein. (region surrounding Ile444)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Background: Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair proteins. Nucleotide excision repair (NER) is the normal cellular response to DNA damage induced by UV irradiation and is disrupted in patients with XP. Xeroderma pigmentosum group A (XPA) is an essential NER factor that coordinates the collection of a preincision complex during the processing of DNA damage. XPA may also have a role in the repair of oxidized DNA bases. XPA is sensitive not only to the structure of the DNA double helix, but also to bulky groups incorporated into DNA. XPA forms a homodimer in the absence of DNA, but binds to DNA in both monomeric and dimeric forms. The dimerically bound XPA is much more efficient, so cells probably regulate XPA activity in a concentration-dependent manner. XPA deficient organisms cannot repair UV-induced DNA damage and thus acquire skin cancers by UV irradiation very easily.Synonyms: VIM

Molecular Weight: approx. 48.0 kDa

Gene ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis