Ubiquitin antibody

Catalog No. ABIN271973

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Ubiquitin protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/20000approx. 1/40000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Ubiquitin antibody (ABIN271973)

Chen, Zhang, Chen, Bao, Wang, Zhu: "Electrical field stimulation induces cardiac fibroblast proliferation through the calcineurin-NFAT pathway." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 12, pp. 1611-22, (2012) (PubMed).

Wang, Zhao, Qi, Gu, Rong, Mu, Zou, Tao, You, Guo: "Gambogic acid-induced degradation of mutant p53 is mediated by proteasome and related to CHIP." in: Journal of cellular biochemistry, Vol. 112, Issue 2, pp. 509-19, (2011) (PubMed).

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Background: Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1, the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IkappaB, p53, cdc25a and Bcl-2 have been shown to be targets for the ubiquitination-proteosome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis.Synonyms: Polyubiquitin-B, UBB

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway