SULF2 antibody

Catalog No. ABIN265174

Product Details anti-SULF2 Antibody

Target: SULF2 (Sulfatase 2 (SULF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SULF2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Sulf2. Antibody tested on human brain lysate.

Purification: Purified

Immunogen: Synthetic peptide derived from the human Sulf2 protein

Isotype: IgG

Application Details

Application Notes: Western blot (0.05-0.1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for SULF2

Target: SULF2 (Sulfatase 2 (SULF2))

Alternative Name: SULF2 (SULF2 Products)

Background: Sulf-2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Mammalian Sulfs are endoproteolytically processed and secreted into the extracellular space of transfected cells, where they exhibit both arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity against intact heparin. Though the molecular weight of full length human Sulf2 is 100455 Da (870 amino acids), the endogenous Sulf2 is found by western blot to be present at molecular weights of 24.4, 33.7 and 51-60 kDa. Higher molecular weight species may be found depending on tissue and/or cell type. Analysis of human tumor tissue and tumor cell lines suggests that HSulf-1 is misregulated in cancers. HSulf-1 is found in a variety of normal tissues but is down-regulated in tumor cell lines originating from ovarian, breast, pancreatic, renal, and hepatocellular carcinoma. Tumors formed by cells expressing Sulf demonstrate enhanced extracellular matrix deposition. The 6-O-sulfation of heparan sulfate of myeloma tumor cells may be a critical factor in determiningand regulating the in vivo growth of this cancer.Synonyms: Extracellular sulfatase Sulf-2, KIAA1247

Gene ID: 55959

NCBI Accession: NP_001155313

UniProt: Q8IWU5