TIRAP antibody (C-Term)

Catalog No. ABIN265134

Product Details anti-TIRAP Antibody

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIRAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts to TIRAP (C-Term).

Purification: Purified

Immunogen: Rabbit polyclonal TIRAP antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of mouse TIRAP.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 to 2 μg/mL. Immunohistochemistry. Found in the cytoplasm and highly expressed in liver, kidney, spleen, skeletal muscle andheart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine,thymus, colon and brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: phosphate buffered saline with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for TIRAP

Target: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Alternative Name: TIRAP (TIRAP Products)

Background: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1 - 3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4,5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kappaB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6,7)Synonyms: Adaptor protein Wyatt, MAL, MyD88 adapter-like protein, TIR domain-containing adapter protein, Toll/interleukin-1 receptor domain-containing adapter protein

Gene ID: 114609

NCBI Accession: NP_001034750

UniProt: P58753

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades