BAX antibody (Isoform beta)

Catalog No. ABIN265017

Product Details anti-BAX Antibody

Target: BAX (BCL2-Associated X Protein (BAX))

Binding Specificity: AA 172-199, Isoform beta

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Immunoprecipitation (IP)

Specificity: This antibody reacts to Bax-beta.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of the Bax beta protein

Isotype: IgG

Application Details

Application Notes: Immunoprecipitation. Will NOT detect denatured protein by Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax (BAX Products)

Background: Bax-beta is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax-beta protein before a stop codon is encountered during translation. The Bax-beta protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax-beta mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax-alpha in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax-beta mRNA was very abundant in brain and duodenum with almost no Bax-alpha mRNA detected in these tissues (Oltvai et al., 1993).Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4

Gene ID: 12028

NCBI Accession: NP_031553

UniProt: Q07813

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response