BNIP3L/NIX antibody (AA 77-92)

Catalog No. ABIN264993

Product Details anti-BNIP3L/NIX Antibody

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Binding Specificity: AA 77-92

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3L/NIX antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes Bnip3L (IN) (nix).

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 77 to 92 of the human Bnip3L protein. Immunogen sequence is identical in the mouse protein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 to 1 μg/mL. Detects a 40 kDa band in K562 cell lysate which representsthe monomer of Bnip3L.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.

References for anti-BNIP3L/NIX antibody (ABIN264993)

Zhang, Randall, Loyd, Dorsey, Kundu, Cleveland, Ney: "Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation." in: Blood, Vol. 114, Issue 1, pp. 157-64, (2009) (PubMed).

Qiu, Jiang, Xiang: "A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 13, pp. 3392-403, (2008) (PubMed).

Marusyk, Wheeler, Mathews, DeGregori: "p53 mediates senescence-like arrest induced by chronic replicational stress." in: Molecular and cellular biology, Vol. 27, Issue 15, pp. 5336-51, (2007) (PubMed).

Aerbajinai, Giattina, Lee, Raffeld, Miller: "The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation." in: Blood, Vol. 102, Issue 2, pp. 712-7, (2003) (PubMed).

Clase, Lyman, del Rio, Randall, Calton, Enquist, Banfield: "The pseudorabies virus Us2 protein, a virion tegument component, is prenylated in infected cells." in: Journal of virology, Vol. 77, Issue 22, pp. 12285-98, (2003) (PubMed).

Target Details for BNIP3L/NIX

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Alternative Name: BNIP3L / BNIP3A (BNIP3L Products)

Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.Synonyms: Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BNIP3H, NIP3-like protein X, NIX

Gene ID: 665

NCBI Accession: NP_004322

UniProt: O60238

Pathways: Autophagy