BAPTA antibody
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- Target
- BAPTA
- Reactivity
- Chemical
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB)
- Purification
- Purified
- Isotype
- IgG
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- Application Notes
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Western Blot: 1 - 10 μg/mL. Immunofluorescence. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- Phosphate buffered saline with 0.08 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- BAPTA
- Target Type
- Chemical
- Background
- BAPTA (bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid)is a calcium channel chelator. BAPTA has spawned many of the most familiar fluorescent indicators for calcium and other ions (1).BAPTA and its analogs including BAPTA-AM have proved most critical in cell physiology where they have been essential in defining the mechanisms underlying intra or intercellular calcium homeostasis (2).and calcium dependant exocytosis (3).BAPTA and its analogs may also find application in diseases which a disturbed regulation of excitatory neurotransmitter release and uptake plays a role (4). The rabbit BAPTA antibody recognizes a 69 kDa monomer and 138 kDa dimer by western blot. The immunogen used was a recombinant BAPTA-KLH.Synonyms: N, N', N'-tetraacetic acid, bis(o-aminophenoxy)-ethane-N, calcium channel chelator
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