HCV E2 antibody (AA 384-665)

Catalog No. ABIN264627

Product Details anti-HCV E2 Antibody

Target: HCV E2 (Hepatitis C Virus Envelope Protein E2 (HCV E2))

Binding Specificity: AA 384-665

Reactivity: Hepatitis C Virus (HCV)

Host: Goat

Clonality: Polyclonal

Conjugate: This HCV E2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Purification: Sodium Sulfate Precipitation and Ion-Exchange Chromatography.

Immunogen: Recombinant E2 (genotype 1a)

Application Details

Application Notes: Conjugation-ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and no stabilizing proteins.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HCV E2

Target: HCV E2 (Hepatitis C Virus Envelope Protein E2 (HCV E2))

Alternative Name: HCV Envelope Glycoprotein E2 (HCV E2 Products)

Target Type: Viral Protein

Background: Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.Synonyms: Hepatitis C Virus NS1, gp68, gp70