Caspase 7 antibody

Catalog No. ABIN264418

Product Details anti-Caspase 7 Antibody

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Reacts with Cysteine-requiring Aspartate Protease-7.

Characteristics: Synonyms: CASP-7, CASP7, MCH3, CMH-1, ICE-like apoptotic protease 3, Apoptotic protease Mch-3

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman caspase-7 protein and mouse myeloma cells. Genename: CASP7

Clone: MCH3-5

Isotype: IgG

Application Details

Application Notes: Western Blot: Detects proenzyme form of human Caspase-7. Positive Control: MCF-7 cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7 (CASP7 Products)

Background: Caspases are key effectors of programmed cell death. Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 is a 303 amino acid protein with high similarity to caspase-3. Caspase-7 occurs in three varient forms. Granzyme B activates pro-caspase-7 to a form which can cleave poly(ADP-ribose) polymerase (PARP) to its signature fragment of ~85 kDa. Also, in vivo caspase-7 appears to be a better substrate for granzyme B than caspase-3. Pro-caspase-7 has been shown to exist as dimers or high order oligomers. Caspase-7 may be an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic T-lymophocyte-induced cell killing in vivo.Synonyms: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3

Gene ID: 840

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity