CD33 antibody (PE)
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- Target See all CD33 Antibodies
- CD33
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD33 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises human Myeloid.
- Characteristics
- Synonyms: Siglec-3, gp67, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3
- Clone
- FOS
- Isotype
- IgG1
- Top Product
- Discover our top product CD33 Primary Antibody
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- Application Notes
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Flow Cytometry. Characterization of subtypes of leukemias and lymphomas. Studies of AIDS virus infection. Myeloid cell function studies. Analysis of hematopoietic maturation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.
- Restrictions
- For Research Use only
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- Buffer
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. - Expiry Date
- 12 months
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- Target
- CD33
- Alternative Name
- CD33 / SIGLEC3 (CD33 Products)
- Synonyms
- SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody
- Background
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is also found on CFU-mix, CFU-GM, CFU-Meg, a portion of BFU-E, myeloblasts, promyelocytes, myelocytes, metamyelocytes but not early precursors.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
- Gene ID
- 945
- UniProt
- P20138
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