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CD5 antibody (APC)

CD5 Reactivity: Human FACS Host: Mouse Monoclonal M28323 APC

Catalog No. ABIN264188

Datasheet as PDF

Target See all CD5 Antibodies

CD5
Reactivity
219

78

53

26

22

16

15

12

11

11

11

9

5

2

1
Human
Host
156

75

50

1
Mouse
Compatible Secondaries
Clonality
213

68
Monoclonal
Conjugate
99

33

28

24

15

8

7

5

5

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD5 antibody is conjugated to APC
Application
219

98

65

62

56

50

30

25

19

15

13

13

12

6

3

2

2

2

2

2

1

1
Flow Cytometry (FACS)

Characteristics

Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5

Clone

M28323

Isotype

IgG2a

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anti-CD5 (CD5) (AA 25-372) antibody
CD5 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IHC, IP, ICC Host: Mouse Monoclonal L17F12 unconjugated

anti-CD5 (CD5) (AA 27-233) antibody
CD5 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 6A11 unconjugated

anti-CD5 (CD5) (AA 403-495) antibody
CD5 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 6C3B2 unconjugated

anti-CD5 (CD5) (AA 269-366) antibody
CD5 Reactivity: Human FACS, IHC, IF, StM Host: Mouse Monoclonal CD5-2418 unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (fro) Host: Mouse Monoclonal CRIS1 unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (p) Host: Mouse Monoclonal MEM-32 unconjugated

anti-CD5 (CD5) antibody (PE)
CD5 Reactivity: Human FACS Host: Mouse Monoclonal L17F12 PE

Application Notes

Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of Bcell subsets, Characterization of subtypes of T cell leukemias and lymphomas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol

PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Restrictions

For Research Use only
Buffer

PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date

12 months
Target

CD5

Alternative Name

CD5 (CD5 Products)

Synonyms

CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background

Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85 % peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID

921

UniProt

P06127