E. Coli antibody
Reactivity: E. coli
WB, EIA, Neut
Host: Mouse
Monoclonal
286-13310
unconjugated
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- Target See all E. Coli products
- E. Coli
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Reactivity
- E. coli
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This E. Coli antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)
- Characteristics
- Synonyms: E. coli SLT-1 alpha and beta
- Purification
- Purified
- Immunogen
- SLT Toxin from E. coli O157: H7
- Clone
- 286-13310
- Isotype
- IgG1
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- Application Notes
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Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157: H7 extracts when used ata 10 μg/mL concentration. Immunoblot: Detected SLT 1 when used at 1 μg/mL concentration. ELISA: Used as capture antibody at 20 μg/mL concentration (100 μL), Immobilizes SLT 1 fordetection by an HRP conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL distilled water
- Concentration
- 1.0 mg/mL (prior to lyophilization)
- Buffer
- 10 mM Phosphate, pH 7.4 containing 0.9 % Sodium chloride.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for two weeksor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- E. Coli
- Abstract
- E. Coli Products
- Target Type
- Bacteria
- Background
- Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.Synonyms: EHEC, EPEC, ETEC, Escherichia coli
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