CD51 antibody

Catalog No. ABIN2624341

Product Details anti-CD51 Antibody

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kD) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kD respectively. NKI-M7 reacts with cultured melanomas melanomas in frozen sections. The antigen detected by NKI-M7 is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. NKI-M7 blocks fibronectin-induced chemotactic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. NKI-M7 stains primary tumors heterogeneously, whereas metastases are homogeneously stained.

Purification: Purified

Immunogen: NKI-M7 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cultured melanoma cells derived from a Human melanoma metastasis.

Type of Immunogen: Cells

Clone: NKI-M7 (former AMF7)

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IP

Usage: CD51 (Cluster of Differentiation 51), known as vitronectin receptor alpha chain, or integrin alpha V is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin beta 1 (CD29), beta 3 (CD61), beta 5, beta 6, or beta 8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its beta chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: ITGAV/Integrin alpha V/CD51 (ITGAV Products)

Background: Name/Gene ID: ITGAV
Family: Integrin

Synonyms: ITGAV, CD51, CD51 antigen, Integrin, alpha V, MSK8, Integrin Alpha V, Integrin alpha-V, VNRA, Vitronectin receptor, Integrin alphaVbeta3, VTNR

Gene ID: 3685

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex