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CD8 alpha antibody (FITC)

CD8A Reactivity: Human FACS Host: Mouse Monoclonal RPA-T8 FITC

Catalog No. ABIN2568027

Datasheet as PDF

Target See all CD8 alpha (CD8A) Antibodies

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Reactivity
180

112

46

45

13

7

4

2

2

1

1

1

1

1

1

1

1
Human
Host
151

124

96
Mouse
Compatible Secondaries
Clonality
269

97

2
Monoclonal
Conjugate
131

41

29

25

24

8

8

8

7

6

5

5

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 alpha antibody is conjugated to FITC
Application
296

114

84

62

61

48

32

20

15

14

13

11

7

6

6

6

5

4

3

2

2

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 α/β) or as a homodimer (CD8 α/α) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells.

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clone

RPA-T8

Isotype

IgG1 kappa

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anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC Host: Rat Monoclonal 53-6-7 unconjugated

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CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 FITC

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CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 PE

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CD8A Reactivity: Human, Rat, Chicken FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody

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CD8A Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 6A9A5 unconjugated

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CD8A Reactivity: Human IHC, StM, ISt Host: Mouse Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reactivity: Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage

4 °C

Storage Comment

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name

CD8a (CD8A Products)

Background

The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 α/β) or as a homodimer (CD8 α/α) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The RPA-T8 antibody reacts with 13-48 % of peripheral lymphocytes, 80 % of thymocytes, and a subset of natural killer cells. RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

Gene ID

925

UniProt

P01732

Pathways

TCR Signaling