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CD8 alpha antibody (violetFluor™ 450)

CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 2-43 violetFluor™ 450

Catalog No. ABIN2567972

Datasheet as PDF

Target See all CD8 alpha (CD8A) Antibodies

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Reactivity
180

112

46

45

13

7

4

2

2

1

1

1

1

1

1

1

1
Mouse
Host
151

124

96
Rat
Compatible Secondaries
Clonality
269

97

2
Monoclonal
Conjugate
131

41

29

25

24

8

8

8

7

6

5

5

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 alpha antibody is conjugated to violetFluor™ 450
Application
296

114

84

62

61

48

32

20

15

14

13

11

7

6

6

6

5

4

3

2

2

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen.

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clone

2-43

Isotype

IgG2b

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anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC, Func Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Mouse FACS, IP, IHC (fro), IHC (p), ICC Host: Rat Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A Reactivity: Mouse FACS Host: Rat Monoclonal 53-6-7 PE

anti-CD8a Molecule (CD8A) (AA 51-150) antibody
CD8A Reactivity: Human, Rat, Chicken FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A Reactivity: Human IHC, StM, ISt Host: Mouse Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A Reactivity: Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.2 mg/mL

Buffer

Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage

4 °C

Storage Comment

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name

CD8a (CD8A Products)

Background

The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen. CD8a (the alpha chains) form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b. CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Gene ID

12525

UniProt

P01731

Pathways

TCR Signaling