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CD3 antibody (FITC)

CD3 Reactivity: Human FACS Host: Mouse Monoclonal UCHT1 FITC

Catalog No. ABIN2567523

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
183

104

26

20

17

10

8

8

7

7

5

3

2

1
Human
Host
218

53

20

13

9

2
Mouse
Compatible Secondaries
Clonality
303

6

1
Monoclonal
Conjugate
96

39

32

23

23

11

9

8

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to FITC
Application
288

82

58

52

46

37

33

23

9

8

7

6

4

3

2

2

2

2

2

2

2

2

1

1

1
Flow Cytometry (FACS)

Specificity

The UCHT1 monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex.

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clone

UCHT1

Isotype

IgG1 kappa

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CD3 Reactivity: Human FACS, IP, Func, CyTOF Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 PerCP

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 APC

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage

4 °C

Storage Comment

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Background

The UCHT1 monoclonal antibody specifically reacts with the ε chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex contains γ, δ, and ε chains, and it is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction. Unlike HIT3a, another specific antibody of CD3, the UCHT1 antibody can stain both the surface and intracellular CD3ε. The immobilized UCHT1 can cross-link with the TCR complex, enhancing cellular activation and proliferation.

Gene ID

916

UniProt

P07766

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway