CD3 antibody (violetFluor™ 450)

Catalog No. ABIN2567483

Product Details anti-CD3 Antibody (violetFluor™ 450)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Specificity: The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation.

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Clone: 17A2

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation. CD3 is expressed by thymocytes and mature lymphocytes, and contains γ, δ, and ε subunits, involved in the assembly, trafficking, and surface expression of T-cell receptor complex. The interaction between the T lymphocytes and the 17A2 antibody can be blocked by the 145-2C11 anti-CD3e antibody, demonstrating that the 17A2 antibody recognizes the CD3 ε chain.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Gene ID: 12501

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway