ATP6V1B1 antibody

Catalog No. ABIN2716450

Product Details anti-ATP6V1B1 Antibody

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP6V1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Characteristics: Homo sapiens ATPase, H+ transporting, lysosomal 56/58 kDa, V1 subunit B1 (ATP6V1B1)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.

Clone: 3B11

Isotype: IgG2b

Application Details

Application Notes: WB 1:2000, IHC 1:150, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ATP6V1B1

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Alternative Name: ATP6V1B1 (ATP6V1B1 Products)

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness.

Molecular Weight: 56.7 kDa

Gene ID: 525

NCBI Accession: NM_001692

HGNC: 525

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport