Fc epsilon RI/FCER1A antibody (AA 1-84) (FITC)

Catalog No. ABIN2451976

Product Details anti-Fc epsilon RI/FCER1A Antibody (FITC)

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Binding Specificity: AA 1-84

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fc epsilon RI/FCER1A antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Characteristics: The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions.

Purification: Purified

Sterility: Sterile filtered

Immunogen: AA 1-84 of FcepsilonR1alpha

Clone: CRA1

Isotype: IgG2b kappa

Application Details

Application Notes: 1) FACS
2) Immunohistochemistry and immunocytochemistry
3) Titration of IgE-bound in combination with CRA2 antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-Fc epsilon RI/FCER1A antibody (FITC) (ABIN2451976)

Suzukawa, Hirai, Iikura, Nagase, Komiya, Yoshimura-Uchiyama, Yamada, Ra, Ohta, Yamamoto, Yamaguchi: "IgE- and FcepsilonRI-mediated migration of human basophils." in: International immunology, Vol. 17, Issue 9, pp. 1249-55, (2005) (PubMed).

Takai, Yuuki, Iwamoto-Yasue, Okumura, Ra: "Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions." in: Bioscience, biotechnology, and biochemistry, Vol. 64, Issue 9, pp. 1856-67, (2001) (PubMed).

Takai, Okumura, Ra: "Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli." in: Bioscience, biotechnology, and biochemistry, Vol. 65, Issue 1, pp. 79-85, (2001) (PubMed).

Yamaguchi, Sayama, Yano, Lantz, Noben-Trauth, Ra, Costa, Galli et al.: "IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5455-65, (1999) (PubMed).

Hakimi, Seals, Kondas, Pettine, Danho, Kochan: "The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22079-81, (1991) (PubMed).

Ra, Jouvin, Blank, Kinet: "A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit." in: Nature, Vol. 341, Issue 6244, pp. 752-4, (1989) (PubMed).

Target Details for Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Abstract: FCER1A Products

Background: FcεR1alpha is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one alpha, one beta and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses. The CRA1 (AER37) monoclonal antibody reacts with the FcεR1alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1alpha, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1alpha.

UniProt: P12319

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process