HA-Tag antibody (AA 98-106)

Catalog No. ABIN243784

Product Details anti-HA-Tag Antibody

Target: HA-Tag

Binding Specificity: AA 98-106

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Sequence: YPYDVPDYA

Specificity: Recognizes HA tagged (YPYDVPDYA) proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.

Purification: Protein A purified

Immunogen: Synthetic peptide containing the sequence aa98-106 (YPYDVPDYA) derived from the influenza virus hemaglutinin (HA) protein (KLH).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG3

Application Details

Application Notes: Approved: ICC (5 μg/mL), IP (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.5-2 μg/mL. Detects recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells. Immunoprecipitation: 5 μg/mL. Reported to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5 μg/mL. Reported to immunostain HA-tagged fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: Hemagglutinin / HA Tag (HA-Tag Products)

Target Type: Tag

Background: Name/Gene ID: HA Tag

Synonyms: HA Tag