CD147 antibody

Catalog No. ABIN238486

Product Details anti-CD147 Antibody

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Characteristics: Synonyms: Basigin, BSG, EMMPRIN, TCSF, Leukocyte activation antigen M6, Collagenase stimulatoryfactor, Extracellular matrix metalloproteinase inducer, 5F7, Tumor cell-derived collagenasestimulatory factor, OK blood group antigen

Purification: Protein-A affinity chromatography.

Clone: MEM-M6-2

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-CD147 antibody (ABIN238486)

Hájková, Bugajev, Dráberová, Vinopal, Dráberová, Janá?ek, Dráber, Dráber: "STIM1-directed reorganization of microtubules in activated mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 913-23, (2011) (PubMed).

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Barinka, Rinnová, Sácha, Rojas, Majer, Slusher, Konvalinka: "Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II." in: Journal of neurochemistry, Vol. 80, Issue 3, pp. 477-87, (2002) (PubMed).

Blaydes, Craig, Wallace, Ball, Traynor, Gibbs, Hupp: "Synergistic activation of p53-dependent transcription by two cooperating damage recognition pathways." in: Oncogene, Vol. 19, Issue 34, pp. 3829-39, (2000) (PubMed).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147 (BSG Products)

Background: CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.Synonyms: 5F7, BSG, Basigin, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins