CD14 antibody (APC)

Catalog No. ABIN238454

Product Details anti-CD14 Antibody (APC)

Target: CD14

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates

Purification: Size-exclusion chromatography

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Clone: MEM-18

Isotype: IgG1

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 10 m l reagent / 100 m l of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (protease free) as a stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody in the dark at 2-8 °C. Do Not freeze! Avoid prolonged exposure to light.
Shelf Life: One year from despatch.

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipo-polysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipo-polysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades