Collagen Type I antibody

Catalog No. ABIN238355

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Specificity: Bovine Collagen type I: 100 % . Bovine Collagen II, IX, XI: < 0.1 % Sheep Collagen type I: 50 % (IHC estimated) Bovine Fibronectin: < 0.1 % Human Collagen type I: < 2 % Chicken Collagen type I: < 0.1 % (Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500). Even though cross-reactivity with human is low in RIA, the antibody has to be expected to be cross-reactiv to human Collagen type I as well as to pig Collagen type I in IHC.

Cross-Reactivity (Details): Species reactivity (tested):Bovine. Cross reacts with Sheep.

Purification: Affinity Chromatography

Immunogen: Purified Collagen type I from Bovine skin

Application Details

Application Notes: RIA. ELISA: 1/400 (OD > 500). Immunoblotting (Western blot). Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissues: 1/500-1/1000. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C overnight. Positive Control: Bovine skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL distilled water. For further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives, None, None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 282187

NCBI Accession: NP_001029211

UniProt: P02453