Borrelia Burgdorferi antibody (FITC)

Catalog No. ABIN236886

Product Details anti-Borrelia Burgdorferi Antibody (FITC)

Target: Borrelia Burgdorferi

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Borrelia Burgdorferi antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reactive Western blot bands at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii.

Characteristics: Rabbit anti B. Burgdorferi, Rabbit antibody to Borrelia burgdorferi (Lyme) Fluorescein conjugated

Purification: Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Whole cell preparation from B. burgdorferi.

Application Details

Application Notes: Suitable for use in direct IFA, Western blot and immunohistochemistry (formalin fixed/paraffin). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Protocol for direct IFA: 1. Multi-well slide contains acetone fixed organisms (BION Enterprises). 2. 2-fold dilutions of ABIN236886 starting at 1:10 out to 1:80 with a counterstain blocking diluents (Evan’s Blue in BSA solution). 3. Incubate 30 minutes at Room Temperature. 4. Wash 5. Mounting medium added to each well before coverslipping the slide. 6. Typically observe 4+ staining for several of the dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH7.2 containing 10 mg/mL BSA, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store (up to 6 months) at 2-8 °C under subdued light. Long term, aliquot and store at -28 °C.

Expiry Date: 6 months

Target Details for Borrelia Burgdorferi

Target: Borrelia Burgdorferi

Alternative Name: Borrelia Burgdorferi (Lyme) (Borrelia Burgdorferi Products)