Nestin antibody

Catalog No. ABIN228953

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Reacts exclusively with the human nestin protein. Species cross-reactivity: Does not cross-react with rodent Nestin.

Purification: Protein G purified

Immunogen: 150-amino-acid fragment from cloned human nestin conjugated to glutathione S-transferase.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC (1:50 - 1:100), IHC, IHC-Fr, IHC-P, WB (1:500 - 1:2500)

Usage: Suitable for use in Immunocytochemistry, Immunohistochemistry, ELISA, and Western blot. Immunohistochemistry: 1:50-1:100. Frozen and paraffin-embedded tissues with a fluorescein or rhodamine conjugated anti-mouse secondary antibody. Immunocytochemistry: 1:50-1:100 with a fluorescein or rhodamine conjugated anti-mouse secondary antibody. Western Blot: 1:500-1:2500 using chemiluminescent detection.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763

UniProt: P48681