Histone H2A antibody (acLys5)

Catalog No. ABIN228323

Product Details anti-Histone H2A Antibody

Target: Histone H2A

Binding Specificity: acLys5

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Recognizes human Acetyl-Histone H2A (Lys5) Antibody detects histone H2A only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic acetyl-peptide KLH coupled) corresponding to residues surrounding Lys5 of human histone H2A.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Histone H2A

Target: Histone H2A

Abstract: Histone H2A Products